Technological Challenges in the Preclinical Development of an HIV Nanovaccine Candidate


Authors: T.G. Dacoba, L. Ruiz-Gatón, A. Benito. M. Klein, D. Dupin, M. Luo, M. Menta, D. Teijeiro-Osorio, I. Loinaz, M.J. Alonso and J. Crecente-Campo

Journal: Drug Delivery and Translational Research

DOI: 10.1007/s13346-020-00721-8

Publication - Abstract

February 10, 2020

Abstract

Despite a very active research in the field of nanomedicine, only a few nano-based drug delivery systems have reached the market. The “death valley” between research and commercialization has been partially attributed to the limited characterization and reproducibility of the nanoformulations. Our group has previously reported the potential of a peptide-based nanovaccine candidate for the prevention of SIV infection in macaques. This vaccine candidate is composed of chitosan/dextran sulfate nanoparticles containing twelve SIV peptide antigens. The aim of this work was to rigorously characterize one of these nanoformulations containing a specific peptide, following a quality-by-design approach. The evaluation of the different quality attributes was performed by several complementary techniques, such as dynamic light scattering, nanoparticle tracking analysis, and electron microscopy for particle size characterization. The inter-batch reproducibility was validated by three independent laboratories. Finally, the long-term stability and scalability of the manufacturing technique were assessed. Overall, these data, together with the in vivo efficacy results obtained in macaques, underline the promise this new vaccine holds with regard to its translation to clinical trials.

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Summary

Microfluidic manufacturing of surface-functionalized graphene oxide nanoflakes for gene delivery

R. Di Santo, L. Digiacomo, S. Palchetti, V. Palmieri, G. Perini, D. Pozzi, M. Papi and G. Caracciolo

Scientists at Sapienza University in Rome report using the NanoAssemblr Benchtop to make Graphene oxide/Cationic lipid (GOCL) hybrid particles for gene delivery. They compared plasmid delivery to standard DOTAP lipoplexes in HEK and HeLa cells and found that transfection efficien...
Read More


Publication - Abstract

In the formulation of nanoparticles, poly(lactic-co-glycolic acid) (PLGA) is commonly employed due to its Food and Drug Administration and European Medicines Agency approval for human use, its ability to encapsulate a variety of moieties, its biocompatibility and biodegradability...
Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Cytiva, formerly Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.