Microfluidic preparation of drug-loaded PEGylated Liposomes, and the Impact of Liposome Size on Tumour Retention and Penetration


Authors: Y.D. Dong, E. Tchung, C. Nowell, S. Kaga, N. Leong, D. Mehta, L.M. Kaminskas and B. Boyd

Journal: Journal of Liposome Research

DOI: 10.1080/08982104.2017.1391285

Publication - Abstract

August 29, 2017

Abstract:

Understanding the effect of liposome size on tendency for accumulation in tumour tissue requires preparation of defined populations of different sized particles. However, controlling the size distributions without changing the lipid composition is difficult, and differences in compositions itself modify distribution behaviour. Here, a commercial microfluidic format as well as traditional methods was used to prepare doxorubicin-loaded liposomes of different size distributions but with the same lipid composition, and drug retention, biodistribution and localization in tumour tissues were evaluated. The small (∼50 nm diameter) liposomes prepared by microfluidics and large (∼75 nm diameter) liposomes displayed similar drug retention in in vitro release studies, and similar biodistribution patterns in tumour-bearing mice. However, the extent of extravasation was clearly dependent on size of the liposomes, with the small liposomes showing tissue distribution beyond the vascular area compared to the large liposomes. The use of microfluidics to prepare smaller size distribution liposomes compared to sonication methods is demonstrated, and allowed preparation of different size distribution drug carriers from the same lipid composition to enable new understanding of tissue distribution in compositionally consistent materials is demonstrated.

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Abstract

Phospholipid‐Free Small Unilamellar Vesicles for Drug Targeting to Cells in the Liver

W. Zhang, R. Böttger, Z. Qin, J.A. Kulkarni, J. Vogler, P.R. Cullis, and S-D. Li

It is reported that cholesterol (Chol) and TWEEN 80 at a molar ratio of 5:1 can form small unilamellar vesicles (SUVs) using a staggered herringbone micromixer. These phospholipid‐free SUVs (PFSUVs) can be actively loaded with a model drug for targeting hepatocytes via the endoge...
Read More


Publication - Abstract

Preparation of Nanoliposomes by Microfluidic Mixing in Herring-bone Channel and the Role of Membrane Fluidity in Liposomes Formation

J. Kotouček, F. Hubatka, J. Mašek, P. Kulich, K. Velínská, J. Bezděková, M. Fojtíková, E. Bartheldyová, A. Tomečková, J. Stráská, D. Hrebík, S. Macaulay, I. Kratochvílová, M. Raška, and J. Turánek

Introduction of microfluidic mixing technique opens a new door for preparation of the liposomes and lipid-based nanoparticles by on-chip technologies that are applicable in a laboratory and industrial scale. This study demonstrates the role of phospholipid bilayer fragment as the...
Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.