Antisolvent Precipitation of Lipid Nanoparticles in Microfluidic Systems – A Comparative Study


Authors: J. Riewe, P. Erfle, S. Melzig, A. Kwade, A. Dietzel, and H. Bunjes

Journal: International Journal of Pharmaceutics

DOI: 10.1016/j.ijpharm.2020.119167

Publication - Abstract

April 15, 2020

Abstract

Microsystems offer promising possibilities to produce nanoparticles which can be used as carriers for poorly water-soluble active substances. The aim of the present study was to compare the preparation of lipid nanoparticles by precipitation in different microsystems: A segmented-flow micromixer, a high-pressure micromixer and the commercial NanoAssemblrTM platform with a staggered herringbone micromixer. A batch set-up served as reference experiment. Castor oil nanoemulsions prepared with polysorbate 80 as surfactant in the aqueous phase were in the size range of 36–160 nm. The particle sizes could be reduced to 43–93 nm when the surfactant was processed via the ethanolic phase. Furthermore, glycerol monooleate nanodispersions (65–141 nm) were manufactured with poloxamer 407 added as stabilizer via the aqueous phase. Deposition of lipid material in the segmented-flow micromixer could be reduced by a modification of the design. Preparation in the high-pressure mixer and in the herringbone mixer at high total flow rates resulted in the smallest particles for castor oil emulsions, but with bimodal distributions. The particle size of glycerol monooleate dispersions was smallest when prepared in the high-pressure micromixer and in the herringbone micromixer at a higher flow rate. In conclusion, microfluidic systems can be a useful tool to produce lipid nanoparticles.

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Abstract

Design of a Novel Vaccine Nanotechnology-based Delivery System Comprising CpGODN-protein Conjugate Anchored to Liposomes

D. Chatzikleanthous, S.T. Schmidt, G. Buffi, I. Paciello, R. Cunliffe, F. Carboni, M.R. Romano, D.T. O'Hagan, U. D'Oro, S. Woods, C.W. Roberts, Y. Perrie, and R. Adamo

Although the well-known Toll like receptor 9 agonist CpGODN has shown promising results as vaccine adjuvant in preclinical and clinical studies, its in vivo stability and potential systemic toxicity remain a concern. In an effort to circumvent these issues, different strategies h...
Read More


Publication - Abstract

Microfluidic devices are mircoscale fluidic circuits used to manipulate liquids at the nanoliter scale. The ability to control the mixing of fluids and the continuous nature of the process make it apt for solvent/antisolvent precipitation of drug-d...

Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.