Get Started
To learn how Precision NanoSystems accelerates nanomedicine development from an idea to clinical applications, contact our Technical Sales Team.
Gene Transfection Kits for Primary Cells
• Up to 95% cell transfection efficiency
• No observed toxicity
• Same reagents in vitro and in vivo
• Easy to use with NanoAssemblr™ technology
• Non-viral transfection
Cell Types:
• Primary Neurons, Astrocytes, and mixed cultures
• iPS derived cells
• Hepatocytes
Nucleic Acids:
• Short interfering RNA (siRNA)
• Messenger RNA (mRNA)
• Plasmid DNA (pDNA)
Transfection Kits
Adapted from RNAi therapeutic technology currently in clinical trials, Precision NanoSystems’ non-viral gene transfection kits perform exceptionally well in cell types that are challenging to transfect with other methods (eg. transfection of primary cells). This technology encapsulates nucleic acids in lipid nanoparticles (LNPs) that mimic low-density lipoproteins (LDLs). LNPs take advantage of an endogenous pathway ubiquitous among mammalian cells to achieve high transfection efficiency (typically >90%) with no observable toxicity. Kits for use with different cell types and nucleic acid payloads including siRNA, messenger RNA (mRNA), and plasmid DNA (pDNA) are available. The kits are designed for use with NanoAssemblr™ systems established in the drug formulation and nanoparticle manufacturing field for ensuring high-quality reproducible results are achieved rapidly with minimal training.
• No observed toxicity
• Same reagents in vitro and in vivo
• Easy to use with NanoAssemblr™ technology
• Non-viral transfection
Cell Types:
• Primary Neurons, Astrocytes, and mixed cultures
• iPS derived cells
• Hepatocytes
Nucleic Acids:
• Short interfering RNA (siRNA)
• Messenger RNA (mRNA)
• Plasmid DNA (pDNA)
Transfection Kits
Adapted from RNAi therapeutic technology currently in clinical trials, Precision NanoSystems’ non-viral gene transfection kits perform exceptionally well in cell types that are challenging to transfect with other methods (eg. transfection of primary cells). This technology encapsulates nucleic acids in lipid nanoparticles (LNPs) that mimic low-density lipoproteins (LDLs). LNPs take advantage of an endogenous pathway ubiquitous among mammalian cells to achieve high transfection efficiency (typically >90%) with no observable toxicity. Kits for use with different cell types and nucleic acid payloads including siRNA, messenger RNA (mRNA), and plasmid DNA (pDNA) are available. The kits are designed for use with NanoAssemblr™ systems established in the drug formulation and nanoparticle manufacturing field for ensuring high-quality reproducible results are achieved rapidly with minimal training.
Above Left: Primary rat neurons expressing GFP 48h after cell transfection with Neuro9-mRNA. Above Right: Primary rat neurons expressing GFP 48h following transfection with Neuro9-pDNA. Bottom: Rendition of the endogenous uptake pathway used by Precision NanoSystems’ transfection reagents to deliver nucleic acids in a non-toxic way.
Simple workflow for Spark Transfection Kit
Simple workflow for NanoAssemblr® Benchtop Transfection Kit
Resource Center
Publication - Abstract
November 29, 2018
International Journal of Pharmaceutics
Rapid and scale-independent microfluidic manufacture of liposomes entrapping protein incorporating in-line purification and at-lin...
Read MorePoster
July 01, 2018
Application Note
July 01, 2018
Publication - Abstract
April 26, 2018
Small Methods
State‐of‐the‐Art Design and Rapid‐Mixing Production Techniques of Lipid Nanoparticles for Nucleic Acid Delivery
Read MorePublication - Abstract
February 27, 2018
Cell Reports
A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing
Read MorePublication - Abstract
November 29, 2018
Carbohydrate Polymers
N-oxy lipid-based click chemistry for orthogonal coupling of mannan onto nanoliposomes prepared by microfluidic mixing...
Read More
MENU
