Liquids in Dishes in a Lab
Small Molecules

Small molecules make up the majority of current pharmaceuticals and are used to treat and manage symptoms of cancer, infections, and depression, among others.


Applications of nanomedicine for small molecule drugs include:

• Drug targeting
• Controlled release
• Improving solubility
• Combination therapy


Small molecule drugs can be formulated into numerous nanoparticle materials. On the market, there are several liposomal formulations of small molecules including Doxil and Visudyne.



Polymeric Nanoparticle and Micelles
Polymer NPs and Micelles


There are however some challenges to producing small molecule-loaded nanoparticles that can be addressed with NanoAssemblr® technology.

Overcome Key Challenges in Making Drug Delivery Nanoparticles

Challenges with 
Conventional Methods
 Benefits of NanoAssemblr® Technology
Formulation affected by production conditionsorangeRightArrowPrecisely controlled formulation conditions
Production is complex and time-consumingorangeRightArrowRapid formulation development
Difficult to scale-up productionorangeRightArrowInherently scalable
Different formulations require different process and equipmentorangeRightArrowProduce a variety of formulations

Key Benefits

Precisely Controlled Formulation Conditions

Laminar flow microfluidic conditions with computer-controlled fluid flow allow conditions of particle precipitation to be precisely controlled.


Precisely Controlled ConditionsPEG-b-PLGA block copolymer micelles of identical size were produced on the NanoAssemblr® Benchtop from polymers of different PLGA block lengths. Typically changing block lengths affect particle size, making it difficult to compare the drug delivery performance of these materials.


With conventional methods, it is difficult to adjust the formulation conditions to produce uniform sizes with different polymers. By contrast, with NanoAssemblr® technology Total Flow Rate (TFR) was adjusted in software to equalize the size of these formulations. The size was determined by DLS of triplicate formulations.

Rapid Formulation Development

Drug loading and particle formation can be performed simultaneously in one step at flow rates typically greater than 10 mL/min. All NanoAssemblr® instruments are intuitive to use requiring no prior experience with microfluidics.


Rapid Formulation DevelopmentSimultaneous drug loading and liposome formation were performed for 4 liposome formulations at a Total Flow Rate of 12 ml/min using a model hydrophobic small molecule drug verteporfin. This enabled rapid identification and optimization of lead formulations.





Inherently Scalable

The NanoAssemblr® process is continuous and can be scaled by flowing more volume through the system, or by using multiple microfluidic mixers in parallel.

Small Molecule: ScaleableLiposome formulations were optimized on the Benchtop and scaled up on the Blaze using a single microfluidic mixer and on the GMP System employing 8 mixers in parallel with identical results. Size and PDI were measured by DLS of triplicate formulations.

Produce a Variety of Formulations

Over 100 papers have demonstrated production of liposomes, polymer NPs, block-co-polymer micelles, emulsions, and lipid nanoparticles.

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To learn how Precision NanoSystems accelerates nanomedicine development from an idea to clinical applications, contact our Technical Sales Team.

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Small Molecule Resources

Publication - Summary

February 07, 2019

Nano Research

Chemotherapy Drugs Derived Nanoparticles Encapsulating mRNA Encoding Tumor Suppressor Proteins to Treat Triple-negative Breast Can...

C. Zhang, X. Zhang, W. Zhao, C. Zeng, W. Li, B. Li, X. Luo, J. Li, J. Jiang, B. Deng, D.W. McComb and Y. Dong

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Publication - Abstract

August 30, 2020

International Journal of Pharmaceutics

Rapid Scale-up and Production of Active-loaded PEGylated Liposomes

C.B. Roces, E.C. Port, N.N. Daskalakis, J.A. Watts, J.W. Aylott, G.W. Halbert and Y. Perrie

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Publication - Abstract

June 11, 2020


Copolymeric Micelles Overcome the Oral Delivery Challenges of Amphotericin B

P. Nimtrakul, D.B. Williams, W. Tiyaboonchai and C.A. Prestidge

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Publication - Abstract

May 02, 2020


Comprehensive Screening of Drug Encapsulation and Co-Encapsulation into Niosomes Produced Using a Microfluidic Device

S. Joshi, R. White, R. Sahu, V.A. Dennis and S.R. Singh

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Publication - Abstract

March 13, 2020


On-Chip Synthesis of Hyaluronic Acid-Based Nanoparticles for Selective Inhibition of CD44+ Human Mesenchymal Stem Cell Proliferati...

E. Chiesa, F. Riva, R. Dorati, A. Greco, S. Ricci, S. Pisani, M. Patrini, T. Modena, B. Conti and I. Genta

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Publication - Abstract

February 26, 2020

International Journal of Molecular Sciences

Dexamethasone Loaded Liposomes by Thin-Film Hydration and Microfluidic Procedures: Formulation Challenges

M.D. Al-Amin, F. Bellato, F. Mastrotto, M. Garofalo, A. Malfanti, S. Salmaso, and P. Caliceti

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