The Self-Assembled Nanoparticle-Based Trimeric RBD mRNA Vaccine Elicits Robust and Durable Protective Immunity Against SARS-CoV-2 in Mice


Authors: W. Sun, L. He, H. Zhang, X. Tian, Z. Bai, L. Sun, L. Yang, X. Jia, Y. Bi, T. Luo, G. Cheng, W. Fan, W. Liu & J. Li

Journal: Signal Transduction and Targeted Therapy

DOI: https://doi.org/10.1038/s41392-021-00750-w

Publication - Abstract

September 09, 2021

In a publication written by scientists, Sun et al. 2021, from the CAS Key Laboratory of Pathogenic Microbiology and Immunology at the Chinese Academy of Sciences, endeavored to develop a broad-spectrum multivalent vaccine to combat rapidly emerging variants of SARS-CoV-2. Their approach involved the production of lipid nanoparticle-based trimeric receptor binding domain (RBD) mRNA vaccines using the NanoAssemblr ® microfluidic mixing platform. The formulation showcased strong immune responses in HACE2-transduced mice that were challenged with SARS-Cov-2. The mRNA payload encoded for the trimeric RBD of the SARS-CoV-2 spike (S) protein and the protein ferritin (TF-RBD) which was then encapsulated by lipid nanoparticle formulations. Aside from the TF-RBD vaccine’s ability to induce robust and durable humoral immunity, several pseudoviral assays applied to immunized mice reveal broad spectrum immunity through higher levels of cross-neutralizing antibody responses against the Alpha, as well as Beta variants of SARS-CoV-2. The versatility and fast engineering approach in developing mRNA payloads to incorporate vaccine adjuvants like ferritin as exemplified in this paper can pave the way for further advancements in nanoparticle subunit vaccines that may play a key role in addressing new viral variants of arising infectious diseases.

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Abstract

Induction of Robust B Cell Responses after Influenza mRNA Vaccination Is Accompanied by Circulating Hemagglutinin-Specific ICOS+ PD-1+ CXCR3+ T Follicular Helper Cells

G. Lindgren, S. Ols, F. Liang, E.A. Thompson, A. Lin, F. Hellgren, K. Bahl, S. John, O. Yuzhakov, K.J. Hassett, L.A. Brito, H. Salter, G. Ciaramella andK. Loré

Modified mRNA vaccines have developed into an effective and well-tolerated vaccine platform that offers scalable and precise antigen production. Nevertheless, the immunological events leading to strong antibody responses elicited by mRNA vaccines a...

Read More


Publication - Abstract

This review will explore the four major pillars required for design and development of an saRNA vaccine: Antigen design, vector design, non-viral delivery systems, and manufacturing (both saRNA and lipid nanoparticles (LNP)). We report on the major innovations, preclinical a...
Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.