Advances in RNAi Therapeutic Delivery to Leukocytes using Lipid Nanoparticles


Authors: S. Ramishetti, D. Landesman-Milo and D. Peer

Journal: Journal of Drug Targeting

DOI: 10.3109/1061186X.2016.1172587

Publication - Abstract

May 04, 2016

Abstract:

Small interfering RNAs (siRNAs) therapeutics has advanced into clinical trials for liver diseases and solid tumors, but remain a challenge for manipulating leukocytes fate due to lack of specificity and safety issues. Leukocytes ingest pathogens and defend the body through a complex network. They are also involved in the pathogeneses of inflammation, viral infection, autoimmunity and cancers. Modulating gene expression in leukocytes using siRNAs holds great promise to treat leukocyte-mediated diseases. Leukocytes are notoriously hard to transduce with siRNAs and are spread throughout the body often located deep in tissues, therefore developing an efficient systemic delivery strategy is still a challenge. Here, we discuss recent advances in siRNA delivery to leukocyte subsets such as macrophages, monocytes, dendritic cells and lymphocytes. We focus mainly on lipid nanoparticles (LNPs) comprised of new generation of ionizable lipids and their ability to deliver siRNA to primary or malignant leukocytes in a targeted manner. Special emphasis is made on LNPs targeted to subsets of leukocytes and we detail a novel microfluidic mixing technology that could aid in changing the landscape of process development of LNPs from a lab tool to a potential novel therapeutic modality.

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Summary

Lipid Nanoparticle Delivery of siRNA to Silence Neuronal Gene Expression in the Brain

R.L. Rungta, H.B. Choi, P.J. Lin, R.W. Ko, D. Ashby, J. Nair, M. Manoharan, P.R. Cullis and B.A. MacVicar

The ability to manipulate gene expression and regulation in neurons is an invaluable asset to advancement of neuroscience and developing therapeutics for neurological disorders. 

Read More


Publication - Abstract

Identifying and Targeting Angiogenesis-related microRNAs in Ovarian Cancer

X. Chen, L.S. Mangala, L. Mooberry, E. Bayraktar, S.K. Dasari, S. Ma, C. Ivan, K.A. Court, C. Rodriguez-Aguayo, R. Bayraktar, S. Raut, N. Sabnis, X. Kong, X. yang, G. Lopez-Berestein, A.G. Lacko, and A. K. Sood

Current anti-angiogenic therapy for cancer is based mainly on inhibition of the vascular endothelial growth factor pathway. However, due to the transient and only modest benefit from such therapy, additional approaches are needed. Deregulation of microRNAs (miRNAs) has been demon...
Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Cytiva, formerly Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.