Expediting in Vitro Characterization of mRNA-Based Gene Therapies via High-Content Fluorescent Imaging


Authors: T.N. Vigila, D. Zhang-Hulsey, J.L. Santos and G.P. Hussmann

Journal: Analytical Biochemistry

DOI: https://doi.org/10.1016/j.ab.2021.114259

Publication - Abstract

August 15, 2021

mRNA Nucleic Acid Lipid Nanoparticles
A study conducted by Vigil et al. 2021 from Astrazeneca Biopharmaceuticals R&D showcases the applicability of high-content fluorescent imaging as a technique for the rapid characterization of mRNA-LNPs generated from a NanoAssemblr® mixing device in order to determine the optimal conditions for transfection that minimize cellular cytotoxicity and maximize mRNA internalization. Through high-content fluorescent imaging, mRNA-LNP characteristics such as transfection parameters, transfection-induced cytotoxicity, mRNA delivery, and mRNA translation were investigated in HEPG2 two dimensional monolayer and three dimensional spheroid cell cultures. This research demonstrates the value in utilizing high fluorescent imaging especially for future studies toward the development of novel gene therapies and drug candidates for various disease areas.

limit search to this category
Advanced Search

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Summary

Sep 24, 2017
Nano Letters

Boosting Intracellular Delivery of Lipid Nanoparticle-Encapsulated mRNA

A. Patel, N. Ashwanikumar, E. Robinson, A. DuRoss, C. Sun, K.E. Murphy-Benenato, C. Mihai, Ö. Almarsson and G. Sahay

mRNA Nucleic Acid Lipid Nanoparticles Genetic Medicine

Gene delivery for the purpose of protein expression has been traditionally performed by electroporation or viral transduction of plasmid DNA. Plasmid delivery has numerous challenges as they could potentially integrate into host genome and require ...

Read More


Publication - Abstract

Mar 16, 2019
Pharmaceutics

What Drives Innovation: The Canadian Touch on Liposomal Therapeutics

A.W.Y. Leung, C. Amador, L.C. Wang, U.V. Mody and M.B. Bally

siRNA Nucleic Acid Lipid Nanoparticles Liposomes
Liposomes are considered one of the most successful drug delivery systems (DDS) given their established utility and success in the clinic. In the past 40–50 years, Canadian scientists have made ground-breaking discoveries, many of which were successfully translated to the c...
Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.
Sign Up