Investigating the Impact of Delivery System Design on the Efficacy of Self-Amplifying RNA Vaccines


Authors: G. Anderluzzi, G. Lou, S. Gallorini, M. Brazzoli, R. Johnson, D.T. O'Hagan, B.C. Baudner and Y. Perrie

Journal: Vaccines

DOI: 10.3390/vaccines8020212

Publication - Abstract

May 08, 2020

mRNA Polymeric Nanoparticles Nucleic Acid Lipid Nanoparticles

Abstract

messenger RNA (mRNA)-based vaccines combine the positive attributes of both live-attenuated and subunit vaccines. In order for these to be applied for clinical use, they require to be formulated with delivery systems. However, there are limited in vivo studies which compare different delivery platforms. Therefore, we have compared four different cationic platforms: (1) liposomes, (2) solid lipid nanoparticles (SLNs), (3) polymeric nanoparticles (NPs) and (4) emulsions, to deliver a self-amplifying mRNA (SAM) vaccine. All formulations contained either the non-ionizable cationic lipid 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or dimethyldioctadecylammonium bromide (DDA) and they were characterized in terms of physico-chemical attributes, in vitro transfection efficiency and in vivo vaccine potency. Our results showed that SAM encapsulating DOTAP polymeric nanoparticles, DOTAP liposomes and DDA liposomes induced the highest antigen expression in vitro and, from these, DOTAP polymeric nanoparticles were the most potent in triggering humoral and cellular immunity among candidates in vivo.

limit search to this category
Advanced Search

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Abstract

Feb 24, 2020
Pharmaceutical Research

Thermodynamic, Spatial and Methodological Considerations for the Manufacturing of Therapeutic Polymer Nanoparticles

S.M. Figueroa, D. Fleischmann, S. Beck and A. Goepferich

Small Molecule Drugs Polymeric Nanoparticles Other Applications
A model water-soluble drug lacking ionizable groups, pirfenidone (PFD), was encapsulated through nanoprecipitation in poly(ethylene glycol)-poly(lactic acid) (PEG-PLA)-poly(lactic-co-glycolic acid) (PLGA) NPs. Firstly, the thermodynamic parameters predicting drug-polymer miscibil...
Read More


Publication - Summary

Jan 14, 2019
Cancer Immunology, Immunotherapy

STAT3 Inhibition Specifically in Human Monocytes and Macrophages by CD163-targeted Corosolic Acid-containing Liposomes

M.N. Andersen, A. Etzerodt, J.H. Graversen, L.C. Holthof, S.K. Moestrup, M. Hokland and H.J. Møller

Targeted Drug Delivery Liposomes Small Molecule Drugs
Researchers from Holger J. Møller's group at Aarhus University in Denmark have published an approach to a cancer immunotherapy that involves altering the function of tumour associated macrophages (TAMs) to fight cancer.  TAMs can take on two main phenotypes: the tumou...
Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Cytiva, formerly Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.
Sign Up