Early Pre-Clinical
Level 1: RNA Vaccines
This course is aimed at providing pharmaceutical scientists with knowledge and confidence to start early preclinical development of RNA-LNP vaccines. The course will be offered in two parts: a live virtual classroom-based part and a live hands-on training portion. Successful completion of both parts is required to receive NanoAssemblr® Ignite™ user certification.
This interactive, live virtual classroom session is designed for bench-based and non-traditional scientists. Fundamentals of RNA-LNP formulations, self-assembly and RNA vaccines will be covered
Registration Fee: US$1000
Module
Learning Objectives
Session Type
Day 1
EPC101
Introduction Module
Understand:
- Advantages and disadvantages of microfluidic nanomedicine production compared with other methods and between different microfluidic approaches
- Molecular mechanisms of bottom-up nanoparticle self-assembly
- Turbulent vs non-turbulent mixing
- How process parameters affect outcomes
- How microfluidic mixers have been used to make various kinds of drug delivery NPs
Tele-Lecture + Live Q&A
2.5 hours
Day 2
EPC102
RNA Vaccine Module
- Review examples from literature of NanoAssemblr use in vaccine development
- Learn importance of the 4 Pillars of RNA vaccine development
- Understand advantages and disadvantages of unmodified, base-modified and self-amplifying vectors
- Guest Lecture Yvonne Perrie: Comparing properties, efficacy, and biodistribution of polymer and lipid-based nanocarriers for RNA vaccines
Tele-Lecture + Video + Live Q&A
1.5 hours
EPC103
Formulation Theory Module
Understand:
- The difference between lipofection and LNPs
- Mechanisms of LNP action and the role of different lipid components
- How different lipids are selected and how they affect physical and performance characteristics
- Limit size
Lecture + Live Q&A
1.5 hours
The practical portion involves live guided instruction designed to familiarize bench-scientists with the complete workflow for preparing RNA-LNPs for early preclinical development. A NanoAssemblr® Ignite™ and other laboratory equipment and supplies are required*. The purchase of a GenVoy-ILM training kit and payload is required, in addition to the course cost. Successful completion of Part I is required.
Registration Fee: US$2200
(Ignite, Training kit and other required laboratory equipment sold separately)
Module
Learning Objectives
Session Type
Day 1
EPC111
NanoAssemblr Operation Module
Understand:
- Become comfortable with the operation of NanoAssemblr Ignite
- Understand Waste
- Learn best practices
Live Guided Hands-On
1 hour
EPC112
Practical: Formulation Workflow
- Be able to complete the workflow for producing RNA-LNPs using GenVoy-ILM including reagent prep, formulation and downstream processing
Live Guided Hands-On
2 hours
Day 2
EPC114
How to Design Experiments with the NanoAssemblr Ignite
- How to design experiments to systematically explore process parameters
- How to design experiments to systematically explore solvents, buffers, reagent concentrations and other formulation parameters
- How to narrow the parameter space and optimize and select lead candidates for late preclinical studies
- Guidelines for in vitro and in vivo dosing for RNA vaccine candidates
Video
0.5 hours
EPC113
Analytical Module 1
- Be able to characterize LNP size by DLS and encapsulation efficiency by LNP-specific Ribogreen assay
Tele-lecture + Video + Live Guidance + Q&A
2.5 hours
Follow up
EPC121 Private Consultation
- Confidential teleconference with PNI Field Applications Scientist to discuss application of course material to specific projects at hand
Teleconference
1 hour
To pre-register, fill in the form, and a Scientific Specialist will be in touch to provide registration information within 2 business days.
Part 1 – Fundamentals $1000
Part 2 – Practical $2200*
*Training kit and payload are required in addition to the course cost. The approximate cost for the training kit and payload is $1600 + shipping and applicable taxes.
Registration now open for the following dates:
Early Pre-clinical Level 1: RNA Vaccines - Part 2 - Practical
FEBRUARY 2022
Early Pre-clinical Level 1: RNA Vaccines - Part 1 - Fundamentals
Feb 8 & 9, 2022 | 9AM-12PM PT
Early Pre-clinical Level 1: RNA Vaccines - Part 2 - Practical
Feb 22 & 23, 2022 | 9AM-12PM PT
MARCH 2022
Early Pre-clinical Level 1: RNA Vaccines - Part 1 - Fundamentals
March 8 & 9, 2022 | 5-8PM PT
Early Pre-clinical Level 1: RNA Vaccines - Part 2 - Practical
March 22 & 23, 2022 | 5-8PM PT
Part II: Successful completion of Part I of this program and access to a NanoAssemblr® Ignite™ is required. NanoAssemblr® Ignite™ is not provided. Please contact a PNI Scientific Specialists to inquire about Ignite. Other required equipment not provided: Dynamic light scattering instrument and consumables, UV absorption spectrometer and consumables, 96-well plate reader and well-plates, micropipettes, a multichannel micropipette, Ribogreen Assay Kit, nuclease-free deionized water. Additionally, training with basic micropipette technique, and safe handling of ethanol or other flammable organic solvents in an appropriate environment is assumed.
Shell Ip, PhD
Dr. Ip is currently the Science Education Manager at Precision Nanosystems has been serving in technical communication and training roles in PNI's commercial team since 2015. He combines his multidisciplinary background with an aptitude for communication to convey complex concepts to diverse audiences through various media. In his postdoctoral and doctoral research, he developed hybrid bio/inorganic nanomaterials for diagnostics applications. He has earned a Ph.D. in Chemistry and a B.A.Sc. in Engineering Science from the University of Toronto.