Early Pre-Clinical
Level 1: RNA Vaccines
This course is aimed at providing pharmaceutical scientists with knowledge and confidence to start early preclinical development of RNA-LNP vaccines. The course will be offered in two parts: a live virtual classroom-based part and a live hands-on training portion. Successful completion of both parts is required to receive NanoAssemblr® Ignite™ user certification.
PRE-REGISTER NOW FOR OUR MAY COURSES
Early Pre-clinical Level 1: RNA Vaccines - Part 1 - Fundamentals
Early Pre-Clinical Level 1: RNA Vaccines - Part 2 - Practical
MORE COURSES
Early Pre-clinical Level 1: RNA Vaccines - Part 1 - Fundamentals
June 1 & 2, 2021
June 29 & 30, 2021
July 27 & 28, 2021
Early Pre-clinical Level 1: RNA Vaccines - Part 2 - Practical
June 8 & 9, 2021
Preview
Module
Learning Objectives
Session Type
EPC101
Introduction to Self-Assembly
Understand:
- Advantages and disadvantages of microfluidic nanomedicine production
- Molecular mechanisms of bottom-up nanoparticle self-assembly
- How process parameters affect outcomes
- How microfluidic mixers have been used to make various kinds of drug delivery NPs
Tele-Lecture + Live Q&A
2h
EPC102
RNA Vaccines
- Review examples from literature of NanoAssemblr use in vaccine development
- Learn the importance of the 4 Pillars of RNA vaccine development
- Understand advantages and disadvantages of unmodified, base-modified and self-amplifying vectors
Guest Lecture Yvonne Perrie: Comparing properties, efficacy, and biodistribution of polymer and lipid-based nanocarriers for RNA vaccines
Tele-Lecture + Video + Live Q&A
1h 30min
EPC103
Fundamentals of LNP Formulation
Understand:
- The difference between lipofection and LNPs
- Mechanisms of LNP action and the role of different lipid components
- How different lipids are selected and how they affect physical and performance characteristics
- How different parameters such as N/P ratios, buffers, etc., affect formulations
Tele-Lecture + Live Q&A
1h 30min
Module
Learning Objectives
Session Type
EPC111
NanoAssemblr Operation
Understand:
- Become comfortable with operation of NanoAssemblr Ignite
- Understand waste
- Learn best practices
Live Remote
Guided Hands-On
15min
EPC112
Formulation Workflow
- Be able to complete the workflow for producing RNA-LNPs using GenVoy-ILM including reagent prep, formulation and downstream processing
Live Remote
Guided Hands-On
2h 15min
EPC114
How to design experiments with the NanoAssemblr Ignite
Learn from expert users:
- How to systematically explore process parameters
- How to systematically explore solvents, buffers, reagent concentrations and other formulation parameters
- How to narrow the parameter space and optimize and select lead candidates for late preclinical studies
- Guidelines for in vitro and in vivo dosing for RNA vaccine candidates
Video
45min
EPC113
Analytical Methods 1
- Be able to characterize LNP size by DLS and encapsulation efficiency by LNP-specific Ribogreen assay
Tele-lecture + Video + Live Guidance
3h
EPC121
Private Consultation
- Confidential teleconference with PNI Field Application Scientist to discuss application of course material to specific projects at hand
Teleconference
Part II: Successful completion of Part I of this program and access to a NanoAssemblr® Ignite™ is required. NanoAssemblr® Ignite™ is not provided. Please contact a PNI Scientific Specialists to inquire about Ignite. Other required equipment not provided: Dynamic light scattering instrument and consumables, UV absorption spectrometer and consumables, 96-well plate reader and well-plates, micropipettes, a multichannel micropipette, Ribogreen Assay Kit, nuclease-free deionized water. Additionally, training with basic micropipette technique, and safe handling of ethanol or other flammable organic solvents in an appropriate environment is assumed.
Shell Ip, PhD
Dr. Ip is currently the Science Education Manager at Precision Nanosystems has been serving in technical communication and training roles in PNI's commercial team since 2015. He combines his multidisciplinary background with an aptitude for communication to convey complex concepts to diverse audiences through various media. In his postdoctoral and doctoral research, he developed hybrid bio/inorganic nanomaterials for diagnostics applications. He has earned a Ph.D. in Chemistry and a B.A.Sc. in Engineering Science from the University of Toronto.
To pre-register, fill in the form, and a Scientific Specialist will be in touch to provide registration information within 2 business days.
Part 1 – Fundamentals $1000
Part 2 – Practical $2200*
*Training kit and payload are required in addition to the course cost. The approximate cost for the training kit and payload is $1600 + shipping and applicable taxes.
Registration now open for May 11 & 12
and
Part 1 – Fundamentals $1000
Part 2 – Practical $2200*
*training kit and payload are required in addition to the course cost. The approximate cost for the training kit and payload is $1600 + shipping and applicable taxes.