NanoMedU

Early Pre-Clinical

Level 1: RNA Vaccines

This course is aimed at providing pharmaceutical scientists with knowledge and confidence to start early preclinical development of RNA-LNP vaccines. The course will be offered in two parts: a live virtual classroom-based part and a live hands-on training portion. Successful completion of both parts is required to receive NanoAssemblr® Ignite™ user certification.

 

 

PRE-REGISTER NOW FOR OUR OCTOBER COURSES

 

 

Early Pre-clinical Level 1: RNA Vaccines - Part 1 - Fundamentals

 

 

Early Pre-Clinical Level 1:  RNA Vaccines - Part 2 - Practical


MORE COURSES

 

Early Pre-clinical Level 1: RNA Vaccines - Part 1 - Fundamentals

 

Nov 9 & 10, 2021

 

Early Pre-clinical Level 1: RNA Vaccines - Part 2 - Practical

 

Nov 23 & 24, 2021

Preview


These excerpts from the course material exemplifies the fundamental molecular-level view that is provided in the Formulation Theory module (EPC103) included in the Early Preclinical Level 1 – RNA Vaccines course
Part I - Fundamentals
This interactive, live virtual classroom session is designed for bench-based and non-traditional scientists. Fundamentals of RNA-LNP formulations, self-assembly and RNA vaccines will be covered
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Module

Learning Objectives

Session Type

Day 1

EPC101

Introduction Module

Understand:

  • Advantages and disadvantages of microfluidic nanomedicine production compared with other methods and between different microfluidic approaches
  • Molecular mechanisms of bottom-up nanoparticle self-assembly
  • Turbulent vs non-turbulent mixing
  • How process parameters affect outcomes
  • How microfluidic mixers have been used to make various kinds of drug delivery NPs

Tele-Lecture + Live Q&A

2.5 hours

Day 2

EPC102

RNA Vaccine Module

  • Review examples from literature of NanoAssemblr use in vaccine development
  • Learn importance of the 4 Pillars of RNA vaccine development
  • Understand advantages and disadvantages of unmodified, base-modified and self-amplifying vectors
  • Guest Lecture Yvonne Perrie: Comparing properties, efficacy, and biodistribution of polymer and lipid-based nanocarriers for RNA vaccines

Tele-Lecture + Video + Live Q&A

1.5 hours

EPC103

Formulation Theory Module

Understand:

 

  • The difference between lipofection and LNPs
  • Mechanisms of LNP action and the role of different lipid components
  • How different lipids are selected and how they affect physical and performance characteristics
  • Limit size

Lecture + Live Q&A

1.5 hours

Part 2 – Practical
The practical portion involves live guided instruction designed to familiarize bench-scientists with the complete workflow for preparing RNA-LNPs for early preclinical development. A NanoAssemblr® Ignite™ and other laboratory equipment and supplies are required*. The purchase of a GenVoy-ILM training kit and payload is required, in addition to the course cost. Successful completion of Part I is required.
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Module

Learning Objectives

Session Type

Day 1

EPC111

NanoAssemblr Operation Module

Understand:

  • Become comfortable with the operation of NanoAssemblr Ignite
  • Understand Waste
  • Learn best practices

Live Guided Hands-On

1 hour

EPC112

Practical: Formulation Workflow

  • Be able to complete the workflow for producing RNA-LNPs using GenVoy-ILM including reagent prep, formulation and downstream processing

Live Guided Hands-On

2 hours

Day 2

EPC114

How to Design Experiments with the NanoAssemblr Ignite

  • How to design experiments to systematically explore process parameters
  • How to design experiments to systematically explore solvents, buffers, reagent concentrations and other formulation parameters
  • How to narrow the parameter space and optimize and select lead candidates for late preclinical studies
  • Guidelines for in vitro and in vivo dosing for RNA vaccine candidates

Video

0.5 hours

EPC113

Analytical Module 1

  • Be able to characterize LNP size by DLS and encapsulation efficiency by LNP-specific Ribogreen assay

Tele-lecture + Video + Live Guidance + Q&A

2.5 hours

Follow up

EPC121 Private Consultation

  • Confidential teleconference with PNI Field Applications Scientist to discuss application of course material to specific projects at hand

Teleconference

1 hour

* NanoAssemblr® Ignite™ is not provided. Please contact a PNI Scientific Specialists to inquire about Ignite. Other required equipment not provided includes: Dynamic light scattering instrument and associated supplies, UV absorption spectrometer and associated supplies, 96-well plate reader and well-plates, micropipettes, a multichannel micropipette, Ribogreen Assay Kit,  nuclease-free deionized water.

Requirements and Prerequisites
Part I: The program is designed to be accessible to an audience with competence in late undergraduate and early graduate level studies in molecular biology, chemistry or related fields.

Part II: Successful completion of Part I of this program and access to a NanoAssemblr® Ignite™ is required. NanoAssemblr® Ignite™ is not provided. Please contact a PNI Scientific Specialists to inquire about Ignite. Other required equipment not provided: Dynamic light scattering instrument and consumables, UV absorption spectrometer and consumables, 96-well plate reader and well-plates, micropipettes, a multichannel micropipette, Ribogreen Assay Kit, nuclease-free deionized water. Additionally, training with basic micropipette technique, and safe handling of ethanol or other flammable organic solvents in an appropriate environment is assumed.

Instructors

Shell Ip, PhD

 

Shell Ip

Dr. Ip is currently the Science Education Manager at Precision Nanosystems has been serving in technical communication and training roles in PNI's commercial team since 2015. He combines his multidisciplinary background with an aptitude for communication to convey complex concepts to diverse audiences through various media. In his postdoctoral and doctoral research, he developed hybrid bio/inorganic nanomaterials for diagnostics applications. He has earned a Ph.D. in Chemistry and a B.A.Sc. in Engineering Science from the University of Toronto.


Pre-Registration

To pre-register, fill in the form, and a Scientific Specialist will be in touch to provide registration information within 2 business days.

 

Part 1 – Fundamentals $1000
Part 2 – Practical $2200*

 

*Training kit and payload are required in addition to the course cost. The approximate cost for the training kit and payload is $1600 + shipping and applicable taxes.

 

 

Registration now open for October 6 & 7, 2021

and

October 20 & 21, 2021

 

 

October 6 & 7, 2021

This course takes place over two days:
Day 1: 7:00 AM - 10:00 AM PT
Day 2: 7:00 AM - 10:00 AM PT

 

Part 1 – Fundamentals $1000

 

October 20 & 21, 2021

This course takes place over two days:

Day 1: 7:00 AM - 10:00 AM PT
Day 2: 7:00 AM - 10:00 AM PT

 

 

Part 2 – Practical $2200*

 

*training kit and payload are required in addition to the course cost. The approximate cost for the training kit and payload is $1600 + shipping and applicable taxes.