Formulation of Non-Ionic Surfactant Vesicles (NISV) Prepared by Microfluidics for Therapeutic Delivery of siRNA into Cancer Cells


Authors: Obeid MA, Elburi A, Young LC, Mullen AB, Tate RJ, Ferro VA

Journal: Molecular Pharmaceutics

DOI: 10.1021/acs.molpharmaceut.7b00352

Publication - Abstract

June 03, 2017

Abstract:

Small interfering RNAs (siRNA) have a broad potential as therapeutic agents to reversibly silence any target gene of interest. The clinical application of siRNA requires the use of safe and effective delivery systems. In this study, we investigated the use of non-ionic surfactant vesicles (NISV) for the delivery of siRNA. Different types of NISV formulations were synthesised by microfluidic mixing and then evaluated for their physiochemical properties and cytotoxicity. The ability of the NISV to carry and transfect siRNA targeting green fluorescent protein (GFP) into A549 that stably express GFP (copGFP-A549) was evaluated. Flow cytometry and Western blotting were used to study the GFP expression knockdown and significant knockdown was observed as a result of siRNA delivery to the cells by NISV. This occurred in particular when using tween 85, which was able to achieve more than 70% GFP knockdown. NISV were thus demonstrated to provide a promising and effective platform for therapeutic delivery of siRNA.

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Blog Posts
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Summary

Researchers from Holger J. Møller's group at Aarhus University in Denmark have published an approach to a cancer immunotherapy that involves altering the function of tumour associated macrophages (TAMs) to fight cancer.  TAMs can take on two main phenotypes: the tumou...
Read More


Publication - Summary

Gaurav Sahay’s lab at Oregon Health and Science University describe a systematic study of lipid nanoparticles (LNPs) for delivering mRNA to the retina. Eleven formulations were made using NanoAssemblr technology and some were administered by subretinal injection in mice. Th...
Read More


Stay Informed

Sign up today to automatically receive new Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.

MENU
菜单
X
X