An mRNA Vaccine Protects Mice against Multiple Tick-Transmitted Flavivirus Infections


Authors: L.A. VanBlarga, S, Himansu, B.M. Foreman, G.D. Ebel, T.C. Pierson and M.S.Diamond

Journal: Cell Reports

DOI: 10.1016/j.celrep.2018.11.082

Publication - Abstract

December 18, 2018

Vaccines Nucleic Acid Lipid Nanoparticles mRNA

Abstract

Powassan virus (POWV) is an emerging tick-transmitted flavivirus that circulates in North America and Russia. Up to 5% of deer ticks now test positive for POWV in certain regions of the northern United States. Although POWV infections cause life-threatening encephalitis, there is no vaccine or countermeasure available for prevention or treatment. Here, we developed a lipid nanoparticle (LNP)-encapsulated modified mRNA vaccine encoding the POWV prM and E genes and demonstrated its immunogenicity and efficacy in mice following immunization with one or two doses. The POWV mRNA vaccine induced high titers of neutralizing antibody and sterilizing immunity against lethal challenge with different POWV strains. The mRNA vaccine also induced cross-neutralizing antibodies against multiple other tick-borne flaviviruses and protected mice against the distantly related Langat virus. These data demonstrate the utility of the LNP-mRNA vaccine platform for the development of vaccines with protective activity against multiple flaviviruses.

limit search to this category
Advanced Search

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Articles
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
    • Cell therapy
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Abstract

Nov 10, 2019
Journal of Controlled Release

Leukocyte-specific siRNA Delivery Revealing IRF8 as a Potential Anti-inflammatory Target

N. Veiga, M. Goldsmith, Y. Diesendruck, S. Ramishetti, D. Rosenblum, E. Elinav, M.A. Behlke, I. Benhar, and D. Peer

Nucleic Acid Lipid Nanoparticles mRNA
Interferon regulatory factor 8 (IRF8) protein plays a critical role in the differentiation, polarization, and activation of mononuclear phagocytic cells. In light of previous studies, we explored the therapeutic potential of IRF8 inhibition as immunomodulatory therapy for inflamm...
Read More


Publication - Summary

Apr 23, 2015
Cell

The Cellular Mechanisms of Neuronal Swelling Underlying Cytotoxic Edema

R.L. Rungta, H.B. Choi, J.R. Tyson, A. Malik, L. Dissing-Olesen, P.J.C. Lin, S.M. Cain, P.R. Cullis, T.P. Snutch and B.A. MacVicar

siRNA Nucleic Acid Lipid Nanoparticles Neuroscience

In this paper, the MacVicar Lab used Neuro9 siRNA cell transfection nanoparticles (Neuro9-siRNA) prepared using the NanoAssemblrTM Benchtop instrument to uncover the role of a specific chloride channel in cytotoxic edema, which is a...

Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Cytiva, formerly Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.
Sign Up