Microfluidic-Based Manufacture of siRNA-Lipid Nanoparticles for Therapeutic Applications


Authors: Walsh C, Ou K, Belliveau NM, Leaver TJ, Wild AW, Huft J, Lin PJ, Chen S, Leung AK, Lee JB, Hansen CL, Taylor RJ, Ramsay EC, Cullis PR

Journal: Methods in Molecular Biology

DOI: 10.1007/978-1-4939-0363-4_6

Publication - Abstract

February 03, 2014

Abstract:

A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles.

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