Publication - Abstract
Nov 29, 2018
International Journal of Pharmaceutics
March 07, 2018
The advancement of nanotechnology toward more sophisticated bioinspired approaches has highlighted the gap between the advantages of biomimetic and biohybrid platforms and the availability of manufacturing processes to scale up their production. Though the advantages of transferring biological features from cells to synthetic nanoparticles for drug delivery purposes have recently been reported, a standardizable, batch‐to‐batch consistent, scalable, and high‐throughput assembly method is required to further develop these platforms. Microfluidics has offered a robust tool for the controlled synthesis of nanoparticles in a versatile and reproducible approach. In this study, the incorporation of membrane proteins within the bilayer of biomimetic nanovesicles (leukosomes) using a microfluidic‐based platform is demonstrated. The physical, pharmaceutical, and biological properties of microfluidic‐formulated leukosomes (called NA‐Leuko) are characterized. NA‐Leuko show extended shelf life and retention of the biological functions of donor cells (i.e., macrophage avoidance and targeting of inflamed vasculature). The NA approach represents a universal, versatile, robust, and scalable tool, which is extensively used for the assembly of lipid nanoparticles and adapted here for the manufacturing of biomimetic nanovesicles.
Publication - Abstract
Nov 29, 2018
International Journal of Pharmaceutics
Publication - Abstract
Mar 18, 2021
European Journal of Pharmaceutics and Biopharmaceutics
Use of the frit-inlet channel improves MD-AF4 analysis of LNP-RNA.
A robust method for AF4 separation of LNP-RNA has been developed and tested on two different platform.
The method meets all the ISO stan...