- An organic solvent containing dissolved lipids and an aqueous solution containing nucleic acids are injected into the two inlet channels of the NanoAssemblr cartridge.
- Under laminar flow, the two solutions do not immediately mix, but microscopic features engineered into the channel cause the two fluids to intermingle in a controlled and reproducible way.
- Within a millisecond, the two fluids are completely mixed, causing a change in solvent polarity that triggers the self-assembly of lipid nanoparticles loaded with nucleic acids.
- Changing the speed and ratio of fluid injection controls the size of the lipid nanoparticles.
- Lipid nanoparticles mimic low-density lipoproteins, which allows them to be taken up by an endogenous cellular transport pathway to deliver nucleic acids to cells.
- Using pH sensitive lipids allow lipid nanoparticles to release encapsulated nucleic acids into the cytoplasm when vesicle pH decreases.
- Lipid Nanoparticles for Short Interfering RNA Delivery
- Immune modulatory nanoparticle therapeutics for intracerebral glioma
- Localized RNAi Therapeutics of Chemoresistant Grade IV Glioma Using Hyaluronan-Grafted Lipid-Based Nanoparticles
- Systemic Gene Silencing in Primary T Lymphocytes Using Targeted Lipid Nanoparticles
- Harnessing RNAi-based nanomedicines for therapeutic gene silencing in B-cell malignancies
- A Glu-urea-Lys Ligand-conjugated Lipid Nanoparticle/siRNA System Inhibits Androgen Receptor Expression In Vivo
- The Cellular Mechanisms of Neuronal Swelling Underlying Cytotoxic Edema
- Lipid Nanoparticle Delivery of siRNA to Osteocytes Leads to Effective Silencing of SOST and Inhibition of Sclerostin In Vivo