Microfluidic Production and Application of Lipid Nanoparticles for Nucleic Acid Transfection


Authors: A. Thomas, S.M. Garg, R.A.G. De Souza, E. Ouellet, G. Tharmarajah, D. Reichert, M. Ordobadi, S. Ip and E.C. Ramsay

Journal: Multiple Myeloma

DOI: 10.1007/978-1-4939-7865-6_14

Publication - Abstract

May 25, 2018

siRNA microRNA DNA

Abstract

Lipid nanoparticles (LNPs) are established in the biopharmaceutical industry for efficient encapsulation and cytosolic delivery of nucleic acids for potential therapeutics, with several formulations in clinical trials. The advantages of LNPs can also be applied in basic research and discovery with a microfluidic method of preparation now commercially available that allows preparations to be scaled down to quantities appropriate for cell culture. These preparations conserve expensive nucleic acids while maintaining the particle characteristics that have made LNPs successful in later stages of genetic medicine development. Additionally, this method and the resulting LNPs are seamlessly scalable to quantities appropriate for in vivo models and development of nucleic acid therapeutics.The present work describes the methodology for preparing LNPs loaded with siRNA, mRNA or plasmids using a commercially available microfluidic instrument and an accompanying transfection kit. Guidelines for application to cultured cells in a well-plate format are also provided.

limit search to this category
Advanced Search

Advanced Search

close
  • Publications
  • Application Notes
  • Posters
  • Workshops
  • Videos & Webinars
  • Blog Posts
Search

Browse by Category

  • Application
    • Diagnostic and Imaging
    • Genetic Medicine
    • Hematology
    • Metabolic Disorders
    • Neuroscience
    • Oncology
    • Skeletal Disorders
    • Targeted Drug Delivery
    • Vaccines
    • Other Applications
  • Formulation
    • Liposomes
    • Nucleic Acid Lipid Nanoparticles
    • Polymeric Nanoparticles
    • Other Formulations
  • Payload
    • DNA
    • microRNA
    • mRNA
    • siRNA
    • Small Molecule Drugs
    • Other Payloads


related content

Publication - Abstract

Apr 25, 2019
Polymers

Polymeric Nanoparticles in Gene Therapy: New Avenues of Design and Optimization for Delivery Applications

R. Rai, S. Alwani and I. Badea

siRNA DNA Polymeric Nanoparticles
The field of polymeric nanoparticles is quickly expanding and playing a pivotal role in a wide spectrum of areas ranging from electronics, photonics, conducting materials, and sensors to medicine, pollution control, and environmental technology. Among the applications of polymers...
Read More


Publication - Summary

Jan 06, 2019
Nanoscale

Microfluidic manufacturing of surface-functionalized graphene oxide nanoflakes for gene delivery

R. Di Santo, L. Digiacomo, S. Palchetti, V. Palmieri, G. Perini, D. Pozzi, M. Papi and G. Caracciolo

DNA Other Formulations Genetic Medicine
Scientists at Sapienza University in Rome report using the NanoAssemblr Benchtop to make Graphene oxide/Cationic lipid (GOCL) hybrid particles for gene delivery. They compared plasmid delivery to standard DOTAP lipoplexes in HEK and HeLa cells and found that transfection efficien...
Read More


Sign Up and Stay Informed
Sign up today to automatically receive new Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.
Sign Up