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Neuro9TM

Transfection

Nucleic Acid Transfection Kits for Primary Neurons

Overview

• 95% cell transfection efficiency
• No observed cytotoxicity
• Gene knockdown via siRNA expression
in vitro and in vivo applications
• Non-viral transfection

 

 

Applications:

• siRNA transfection for gene knockdown via RNAi both in vitro and in vivo

 

Top Right: Enriched (>90%) E18 primary rat cortical neurons treated at DIV12 with Neuro9 siRNA against HPRT. HPRT mRNA assayed by qPCR 48h post-treatment. *** P < 0.001

 

Middle Right: Enriched (>90%) E18 primary rat cortical neurons treated at DIV7 with Neuro9 siRNA against HPRT for 48 hours. Cell viability assayed using PrestoBlue.

 

Bottom Right: Primary rat neurons were treated at various stages of maturity with Neuro9 siRNA against PTEN. Neuro9 is well tolerated and can be applied at DIV ≥ 2. This flexibility opens doors to new experimental possibilities. *** P < 0.001

90% knockdown in 95% of Cells
90% Knockdown in 95% of Cells

No Observable Toxicity
No Observable Toxicity

Mediate Gene Expression as Early as DIV2
Mediate Gene Expression Early

Neuro9 in vitro cell culture and transfection
siRNA : > 95% Cellular Transfection Efficiency
Neuro9 In Vitro Cell Culture & Transfection

Images: Fluorescently labeled Neuro9 (red) is used to observe uptake by MAP2+ neurons (green).

Plot: Flow cytometry indicates Neuro9 uptake in >95% of cells


Neuro9 in vivo
siRNA : > 95% Transfection Efficiency
Neuro9 In Vivo Transfection
Neuro9 (red) was locally injected into rat somatosensory cortex. Brains were removed 5 days later, sliced and imaged. Calcein-AM dye (green) indicates neurons were alive when imaged. Western blots from samples 1mm from the injection site exhibit significant knockdown of target gene PTEN compared to “control” samples taken from the opposite hemisphere and samples treated with Luc siRNA.

Neuro9 Spark
Transfection Kits
Kit TypeEncapsulatesTreats (wells of 24 well plate)Catalogue
siRNA2 nmol (2x)50NWS0001
siRNA5 nmol100NWS0002

Neuro9 Benchtop
Transfection Kits
Kit TypeEncapsulatesTreats (wells of 24 well plate)Catalogue
siRNA0.5 mg15NWT0001
siRNA2 mg20NWT0002

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Resource Center

Publication - Abstract

April 02, 2020

Journal of Controlled Release

Design of a Novel Vaccine Nanotechnology-based Delivery System Comprising CpGODN-protein Conjugate Anchored to Liposomes

D. Chatzikleanthous, S.T. Schmidt, G. Buffi, I. Paciello, R. Cunliffe, F. Carboni, M.R. Romano, D.T. O'Hagan, U. D'Oro, ...

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Publication - Abstract

December 31, 2019

Vaccines

Investigating Prime-Pull Vaccination through a Combination of Parenteral Vaccination and Intranasal Boosting

C.B. Roces, M.T. Hussain, S.T. Schmidt, D. Christensen and Y. Perrie

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Publication - Abstract

May 31, 2019

Vaccine

mRNA Vaccines Against H10N8 and H7N9 Influenza Viruses of Pandemic Potential Are Immunogenic and Well Tolerated in Healthy Adults ...

R.A. Feldman, R. Fuhr, I. Smolenov, A. Ribeiro, L. Panther, M. Watson, J.J. Senn, M. Smith, Ö. Almarsson, H.S. Pujar, M....

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Publication - Summary

May 18, 2019

Annals of Hematology

Lipid nanoparticle-mediated siRNA delivery for safe targeting of human CML in vivo

N. Jyotsana, A. Sharma, A. Chaturvedi, et al.

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Publication - Summary

May 17, 2019

Science Immunology

A lipid-encapsulated mRNA encoding a potently neutralizing human monoclonal antibody protects against chikungunya infection

N. Kose, J.M. Fox, G. Sapparapu, et al.

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Publication - Summary

April 04, 2019

The Journal of Neuroscience

PTCD1 is required for mitochondrial oxidative-phosphorylation: possible genetic association with Alzheimer's disease

D. Fleck, L. Phu, E. Verschueren, T. Hinkle, M. Reichelt, T. Bhangale, B. Haley, Y. Wang, R. Graham, D.S. Kirkpatrick, M...

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