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Neuro9TM

Transfection

Nucleic Acid Transfection Kits for Primary Neurons

Overview
• 95% cell transfection efficiency
• No observed cytotoxicity
• Gene knockdown via siRNA and mRNA and pDNA expression
• in vitro and in vivo applications
• Non viral transfection

Applications:

in vitro
• siRNA transfection for gene knockdown via RNAi
• mRNA transfection for transient exogenous gene expression
• plasmid DNA transfection for exogenous gene expression

in vivo
• siRNA transfection for gene knockdown via RNAi


Top Right: Enriched (>90%) E18 primary rat cortical neurons treated at DIV12 with Neuro9 siRNA against HPRT. HPRT mRNA assayed by qPCR 48h post-treatment. *** P < 0.001

Middle Right: Enriched (>90%) E18 primary rat cortical neurons treated at DIV7 with Neuro9 siRNA against HPRT for 48 hours. Cell viability assayed using PrestoBlue.

Bottom Right: Primary rat neurons were treated at various stages of maturiny with Neuro9 siRNA against PTEN. Neuro9 is well tolerated, and can be applied at DIV ≥ 2. This flexibilty opens doors to new experimental possibilities. *** P < 0.001

90% knockdown in 95% of Cells
Kits-Fig1@2x

No Observable Toxicity
NoTox

Mediate Gene Expression as Early as DIV2
NoTox

Neuro9 in vitro cell culture and transfection
siRNA : > 95% Cellular Transfection Efficicency
Spark Transfection Kit Workflow
Images: Fluorescently labeled Neuro9 (red) is used to observe uptake by MAP2+ neurons (green).
Plot: Flow cytometry indicates Neuro9 uptake in >95% of cells

Neuro9 in vivo
siRNA : > 95% Transfection Efficicency
NABT-workflow-2x
Neuro9 (red) was locally injected into rat somatosensory cortex. Brains were removed 5 days later, sliced and imaged. Calcein-AM dye (green) indicates neurons were alive when imaged. Western blots from samples 1mm from the injection site exhibit significant knockdown of target gene PTEN compared to “control” samples taken from the opposite hemisphere and samples treated with Luc siRNA.

Neuro9 Spark
Transfection Kits
Kit TypeEncapsulatesTreats (wells of 24 well plate)DetailsCatalogue Request
siRNA2 nmol (2x)50Spec SheetNWT0001Request Information
siRNA5 nmol100Spec SheetNWT0002Request Information

Neuro9 Benchtop
Transfection Kits
Kit TypeEncapsulatesTreats (wells of 24 well plate)DetailsCatalogue Request
siRNA0.5 mg15Spec SheetNWT0001Request Information
siRNA2 mg20Spec SheetNWT0002Request Information

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Resource Center

Poster

July 01, 2018

Robust low-volume production of nanoparticles for genetic manipulation of cells

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Application Note

July 01, 2018

Seamless scale up of liposomal verteporfin formulations using the NanoAssemblr™ Platform

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Publication - Abstract

April 26, 2018

Small Methods

State‐of‐the‐Art Design and Rapid‐Mixing Production Techniques of Lipid Nanoparticles for Nucleic Acid Delivery

Evers, M. J. W., Kulkarni, J. A., van der Meel, R., Cullis, P. R., Vader, P., & Schiffelers, R. M.

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Publication - Abstract

February 27, 2018

Cell Reports

A Single Administration of CRISPR/Cas9 Lipid Nanoparticles Achieves Robust and Persistent In Vivo Genome Editing

J. Finn, A. Smith, M. Patel, L. Shaw, M. Youniss, J. Heteren, T. Dirstine, C. Ciullo, R. Lescarbeau, J. Seitzer, R. Shah...

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Publication - Abstract

July 04, 2018

Nature communications

Rapid transport of deformation-tuned nanoparticles across biological hydrogels and cellular barriers

M Yu, L Xu, F Tian, Q Su, N Zheng, Y Yang

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Publication - Abstract

June 15, 2018

Molecular therapy

Lipid Nanoparticle-Delivered Chemically Modified mRNA Restores Chloride Secretion in Cystic Fibrosis

E Robinson, KD MacDonald, K Slaughter, M McKinney

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Resource Center

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