Scientist Holding Small Formulation

Liposome Components DiagramLiposomes are drug delivery vehicles that can be formulated with a wide variety of natural, synthetic, and modified lipid species to deliver drugs and contrast agents. There are 15 liposomal drug formulations on the market (as of 2017) for indications such as cancer, fungal infections, macular degeneration, pain management and vaccines. There are many applications using liposomes in clinical, preclinical, and basic research, including:


• Drug delivery
• Gene delivery/transfection
• Contrast agents for medical imaging (eg: ultrasound, MRI
and fluorescence)
• Precursors for lipid coatings (eg: biosensors)
• Model cell membranes

Key Benefits    How it works    Resources

Overcome Key Challenges in Advancing Liposome Formulations

Challenges with conventional methods  Solutions with the NanoAssemblr Platform
The formulation process has significant batch-to-batch variabilityorangeRightArrowReproducible liposome manufacturing conditions
Maintaining precise control over the particle size is difficultorangeRightArrowControl liposome size through instrument parameters
Loading liposomes requires multiple steps orangeRightArrowCombined drug loading and liposome formation in one step for efficient and versatile drug loading
Production is time-consuming and labor-intensiveorangeRightArrow
Rapid, effortless liposome production and optimization
The manufacturing process is difficult to scale-uporangeRightArrowA seamless path to scaling up production

Key Benefits

Reproducible Liposome Manufacturing Conditions

Liposome Reproducibility GraphLiposome size and polydispersity were consistent across six independent batches prepared by different operators.

Liposome Size Control Through Convenient Instrument Parameters

Liposome Size Control FRR
The effects of adjusting Total Flow Rate (TFR) and Flow Rate Ratio (FRR) in the NanoAssemblr® software on nanoparticle size. Parameters can be easily adjusted to achieve a specific size for a given lipid composition.

Combine Drug Loading and Liposome Formation in One Step for Efficient and Versatile Drug Loading

Rapid Formulation Development
Encapsulation efficiency of verteporfin loaded liposomes containing natural (egg-PC, soy-PC) or synthetic phospholipids (POPC, DSPC) as the primary lipid component.

A Seamless Path to Scaling Up Production

Small Molecule Scaleable
An example shown here is a liposome formulation (POPC/Cholesterol 60/40 mol/mol), optimized using the NanoAssemblr Benchtop and scaled up using the NanoAssemblr® Blaze™ and the GMP System with minimal process development and identical results.

How it works

NanoAssemblr Microfluidic Mixing

An organic solvent with dissolved lipids and an aqueous solution are injected into the two inlets of the NanoAssemblr® mixer. With laminar flow, the two solutions do not immediately mix. Microscopic features engineered into the channels cause the two fluids to intermingle in a controlled and reproducible way. Within 1 ms, the two fluids are completely mixed. This mixing causes a change in solvent polarity which triggers self-assembly of lipids into liposomes. Controlling the speed and ratio of fluid injection controls the size of the liposomes.

Get Started

To learn how Precision NanoSystems accelerates nanomedicine development from an idea to clinical applications, contact our Technical Sales Team.

Get in Touch

Liposome Resources

Publication - Abstract

May 08, 2020


Investigating the Impact of Delivery System Design on the Efficacy of Self-Amplifying RNA Vaccines

G. Anderluzzi, G. Lou, S. Gallorini, M. Brazzoli, R. Johnson, D.T. O'Hagan, B.C. Baudner and Y. Perrie

Read More 阅读更多

Publication - Abstract

April 02, 2020

Journal of Controlled Release

Design of a Novel Vaccine Nanotechnology-based Delivery System Comprising CpGODN-protein Conjugate Anchored to Liposomes

D. Chatzikleanthous, S.T. Schmidt, G. Buffi, I. Paciello, R. Cunliffe, F. Carboni, M.R. Romano, D.T. O'Hagan, U. D'Oro, ...

Read More 阅读更多

Publication - Abstract

August 30, 2020

International Journal of Pharmaceutics

Rapid Scale-up and Production of Active-loaded PEGylated Liposomes

C.B. Roces, E.C. Port, N.N. Daskalakis, J.A. Watts, J.W. Aylott, G.W. Halbert and Y. Perrie

Read More 阅读更多

Publication - Abstract

July 19, 2020

Current Topics in Microbiology and Immunology

Advances in Development of mRNA-Based Therapeutics

L. Huang, L. Zhang, W. Li, S. Li, J. Wen, H. Li and Z. Liu

Read More 阅读更多

Publication - Abstract

July 08, 2020

Advanced Drug Delivery Reviews

Liposomes: Advancements and Innovation in the Manufacturing Process

S. Shah, V. Dhawan, R. Holm, M.S. Nagarsenker and Y. Perrie

Read More 阅读更多

Publication - Abstract

June 15, 2020

Chemical Engineering Journal

Microfluidic Synthesis Control Technology and Its Application in Drug Delivery, Bioimaging, Biosensing, Environmental Analysis and...

C.T. Kung, H. Gao, C.Y. Lee, Y.N. Wang, W. Dong C.H. Ko, G. Wang and L.M. Fu

Read More 阅读更多
Resource Center 资源中心

Stay Informed

Sign up today to automatically receive new Precision NanoSystems application notes, conference posters, relevant science publications, and webinar invites.